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Regulation of the IL-33/ST2 pathway contributes to the anti-inflammatory effect of acupuncture in the ovalbumin-induced murine asthma model
  1. Ming Dong1,
  2. Cheng Ma2,3,
  3. Wen-Qian Wang2,3,
  4. Juan Chen4,
  5. Ying Wei2,3
  1. 1Gumei Community Health Center of Minhang District of Shanghai, Shanghai, China
  2. 2Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, China
  3. 3Institutes of Integrative Medicine of Fudan University, Shanghai, China
  4. 4Department of Pediatric Neurological Rehabilitation, Maternal and Child Health Hospital of Dengfeng, Henan, China
  1. Correspondence to Professor Ying Wei, Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China; weiying_acup{at}126.com

Abstract

Background Bronchial asthma is a chronic airway inflammatory disease which has three main pathological features: airway hyperresponsiveness (AHR), airway remodelling, and chronic inflammation. Acupuncture is known to be an effective integrative medical therapy that has been used in the treatment of several chronic diseases, including bronchial asthma. The aim of the current study was to evaluate the effects of acupuncture on inflammation and regulation of the IL-33/ST2 pathway in a mouse model of asthma.

Methods The murine asthma model was established by both injection and inhalation of ovalbumin (OVA). Within 24 hours of the last OVA challenge, lung function was assessed by measurement of the airway resistance (RL) and lung dynamic compliance (Cdyn). Pulmonary tissues were collected for the detection of pathological changes and mucus secretion. Serum levels of tumour necrosis factor α (TNF-α), interleukin (IL)-1β, IL-33 and sST2 (secreted ST2) were detected by ELISA. Th17 cell proportions and counts in bronchoalveolar lavage fluid (BALF) were analysed by flow cytometry.

Results The results showed that AHR, chronic inflammation and mucus secretion were significantly suppressed by acupuncture treatment. RL decreased while Cdyn increased after acupuncture treatment. There was an apparent decrease in the serum concentrations of certain pro-inflammatory cytokines, such as TNF-α, IL-1β and IL-33, and an increase in sST2 level compared with untreated asthmatic mice. Acupuncture also reduced the CD4 +IL-17A+ cell proportion and counts in BALF.

Conclusion Acupuncture effectively protects lung function and attenuates airway inflammation in the OVA-induced mouse model of asthma, which supports the role of acupuncture as a potential therapy in asthma treatment.

  • acupuncture
  • immunology
  • asthma
  • chronic airways disease

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Footnotes

  • MD and CM contributed equally.

  • Contributors YW conceived and designed the study. MD and CM wrote the manuscript. MD established the animal model of asthma and administered the acupuncture treatment. CM performed the AHR detection and pulmonary histopathology analysis. WW and JC performed the Bio-Plex and ELISA assays. CM, WW and YW analysed the data and revised the manuscript. All the authors read and approved the final version of the manuscript accepted for publication, and agree to be accountable for all aspects of the work.

  • Funding This work was supported by the National Natural Science Foundation of China (ref: 81403476), Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine (ref: 20150407), Shanghai Health and Family Planning Commission Program for Traditional Chinese Medicine (ref: 2016JP001), scientific research project of Shanghai Science and Technology Commission (ref: 17401930300), Shanghai Health and Family Planning Commission Program of Minhang district (ref: 2016MW42) and Shanghai Science and Technology Commission Program of Minhang district (ref: 2017MHZ61) .

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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