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Effects of acupuncture at ST25 on inflammatory mediators and nuclear factor κB activation in a rat model of severe acute pancreatitis
  1. Qi-Ming Xue,
  2. Hui Pan,
  3. Lu Huang,
  4. Ning Li
  1. Department of Integrated Chinese and Western Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan Province, China
  1. Correspondence to Professor Ning Li, Integrated Chinese and Western Medicine Department, West China Hospital of Sichuan University, 37 # Guoxue Lane, Chengdu, Sichuan Province 610041, China; zhenjiuhuaxi{at}163.com

Abstract

Objective To observe the effect of electroacupuncture (EA) and manual acupuncture (MA) at ST25 on inflammatory mediators and nuclear factor κ-B (NF-κB) activation in rats with sodium taurocholate-induced severe acute pancreatitis (SAP).

Methods Eighty-eight male Sprague–Dawley rats were randomly divided into four groups: control (sham-operated), SAP, SAP+EA and SAP+MA (n=22 each). A SAP model was established by injecting 3.5% sodium taurocholate 1 mL/kg into the pancreatic duct. In each group, animals were killed at t=3 h (n=7), 6 h (n=7) and 12 h (n=8) after the procedure. Pancreatic expression of NF-κB was examined by immunohistochemical staining. The levels of tumour necrosis factor α (TNF-α) and interleukin 6 (IL-6) in serum were determined by ELISA. Pathological changes in the pancreas were examined microscopically.

Results Serum levels of TNF-α and IL-6 increased and morphological changes consistent with tissue damage were observed in the pancreas of SAP rats. NF-κB p65 expression was significantly higher in the SAP group than in the sham-operated group (p<0.05). Treatment with acupuncture at ST25 attenuated morphological damage and reduced levels of TNF-α and IL-6 in serum. NF-κB p65 expression was also significantly reduced by acupuncture at ST25 in the pancreas at 6 and 12 h after the procedure (p<0.05). There were no significant differences between the SAP+EA and SAP+MA groups.

Conclusions Acupuncture at ST25 might have a therapeutic effect on rats with SAP through inhibition of NF-κB expression and a reduction in the release of pro-inflammatory cytokines.

  • ACUPUNCTURE

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