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Effects of electroacupuncture at ST25 and BL25 in a Sennae-induced rat model of diarrhoea-predominant irritable bowel syndrome
  1. Xianwei Zhu1,
  2. Zhibin Liu1,2,
  3. Wenmin Niu1,2,
  4. Yuan Wang1,
  5. Aimin Zhang3,
  6. Hongyan Qu2,
  7. Jing Zhou4,
  8. Lu Bai5,
  9. Yong Yang6,
  10. Jie Li2
  1. 1Innovation Research Centre of Acupuncture Combined with Medicine, Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Ecomic Zone, Xi'an City, Shaanxi Province, China
  2. 2Department of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Ecomic Zone, Xianyang City, Shaanxi Province, China
  3. 3Department of Urologic Surgery, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Ecomic Zone, Xi'an City, Shaanxi Province, China
  4. 4College of Public Hygiene, Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Ecomic Zone, Xianyang, Shaanxi Province, China
  5. 5Department of English, Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Ecomic Zone, Xi'an, Shaanxi Province, China
  6. 6College of Basic Medicine, Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Ecomic Zone, Xianyang, Shaanxi Province, China
  1. Correspondence to Dr Zhibin Liu, Department of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Ecomic Zone, Xianyang City, Shaanxi Province 712046, China; jater0228{at}outlook.com, lzb210396{at}163.com

Abstract

Background Electroacupuncture (EA) may have a role in the treatment of diarrhoea symptoms. Serotonin (5-hydroxytryptamine, 5-HT) is an important neurotransmitter and paracrine signalling molecule in the gastrointestinal (GI) tract, which initiates peristaltic, secretory, vasodilatory, vagal and nociceptive reflexes. In addition, according to the results of our previous report, EA stimulation mediates GI peristalsis by increasing expression of 5-HT and tryptophan hydroxylase (TPH).

Aim To investigate the effect of EA at acupuncture points ST25 and BL25 in a rat model of diarrhoea.

Methods A diarrhoea-predominant irritable bowel syndrome (IBS-D) model was induced by Folium Sennae in 24 rats, which remained untreated (n=6) or received EA at ST25 (n=6), BL25 (n=6) or the combination of ST25 and BL25 (n=6). A control group of healthy rats was also included (n=6). After treatment, changes in loose stool and small intestine transit rates, enterochromaffin (EC) cell number, expression of TPH, and faecal/colonic 5-HT contents were measured.

Results Loose stool and small intestine transit rates, EC cell numbers, colonic TPH expression and faecal/colonic 5-HT content of IBS-D rats were significantly increased relative to controls (p<0.05) and all these parameters were improved by EA at ST25, BL25, or ST25 and BL25 in combination (all p<0.05 vs untreated IBS-D rats).

Conclusions EA at ST25 and/or BL25 had a positive effect on objective markers of diarrhoea in a IBS-D rat model and induced changes in EC cell number, colonic TPH and 5-HT contents. The effects of EA stimulation at ST25/BL25 on IBS-D rats may be mediated by excitation of sympathetic nerves.

  • GASTROENTEROLOGY)

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Footnotes

  • Contributors Dr Xianwei Zhu and Dr Zhibin Liu designed the study protocol and performed the experiment. Dr Wenmin Niu, Dr Jing Zhou, Dr Yuan Wang, Mr Yong Yang and Mr Jie Li participated in the discussion and helped with the experiment. Ms Aimin Zhang, Ms Hongyan Qu and Ms Lu Bai prepared the manuscript. All authors approved the final version accepted for publication.

  • Funding This study was supported by grants from the National Basic Research Program of China (973 Program, grant number 2011CB505204).

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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